MMP9 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB1294373
Article Name: MMP9 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB1294373
Supplier Catalog Number: orb1294373
Alternative Catalog Number: BYT-ORB1294373-25,BYT-ORB1294373-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human
Immunogen: Synthetic peptide / encompassing a sequence within the center region
Conjugation: Unconjugated
Rabbit polyclonal antibody to MMP 9
Clonality: Polyclonal
Buffer: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target: MMP9
Application Dilute: Western Blot 1:500, Immunofluorescence 1:200-1:500, Immunohistochemistry (Paraffin) 1:100-1:200
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294373 anti-MMP9 antibody (red) at 1:250 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling MMP9 with orb1294373 at 1/100.
Immunohistochemical analysis of paraffin embedded Human Colon cancer tissue labeling MMP9 with orb1294373 at 1/100.
Immunohistochemical analysis of paraffin embedded Human kidney cancer tissue labeling MMP9 with orb1294373 at 1/100.
Immunohistochemical analysis of paraffin embedded Human kidney cancer tissue labeling MMP9 with orb1294373 at 1/100.
MMP9 antibody - at 1/500 dilution. Lysates at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294373 overnight at 4C.
MMP9 antibody - at 1/500 dilution. Lysates at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294373 overnight at 4C.