Synthetic peptide / encompassing a sequence within the C-terminus region.
Conjugation:
Unconjugated
Rabbit polyclonal antibody to Beta Actin
Clonality:
Polyclonal
Buffer:
100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target:
Beta-actin
Application Dilute:
Western Blot 1:2000-1:5000, Immunofluorescence 1:600-1:700, Immunohistochemistry (Paraffin) 1:400-1:600
Anti-Beta Actin antibody - at 1/2000 dilution, Lysates at 40 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294415 overnight at 4C.
Immunofluorescence: cells were fixed with 4% PFAfor 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294415 anti-Beta Actin antibody (red) at 1:400 and 4C.
Immunofluorescence: cells were fixed with 4% PFAfor 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294415 anti-Beta Actin antibody (red) at 1:400 and 4C.
Immunofluorescence: cells were fixed with 4% PFAfor 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294415 anti-Beta Actin antibody (red) at 1:400 and 4C.
Immunofluorescence: cells were fixed with 4% PFAfor 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294415 anti-Beta Actin antibody (red) at 1:400 and 4C.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Beta Actin with orb1294415 at 1/600.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Beta Actin with orb1294415 at 1/600.
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