0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
CLEC7A
Application Dilute:
WB=1:500-2000, Flow-Cyt=1ug/Test
WB analysis of Raji lysate using Dectin 1 antibody (dilution at 1:300)
Blank control: RAW264.7. Primary Antibody (green line): Rabbit Anti-Iba1 antibody (orb13296), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-Rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-beta Glucan Receptor antibody (orb13296), Dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Sample: Lane 1: Mouse Spleen tissue lysates, Lane 2: Mouse Lung tissue lysates, Primary: Anti-beta Glucan Receptor (orb13296) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 28 kDa, Observed band size: 28, 47 kDa.
Sample: Lung (Mouse) lysate at 30 ug, Primary: Anti-beta Glucan Receptor (orb13296) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 28 kD, Observed band size: 28 kD.
Sample: Spleen (Mouse) Lysate at 40 ug, Thymus (Mouse) Lysate at 40 ug, Primary: Anti-beta Glucan Receptor (orb13296) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 28 kD, Observed band size: 28 kD.
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