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Ki-67 Rabbit Polyclonal Antibody, Unconjugated

Ki-67 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB13523

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Article Name:	Ki-67 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number:	BYT-ORB13523
Supplier Catalog Number:	orb13523
Alternative Catalog Number:	BYT-ORB13523-50,BYT-ORB13523-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	FC, ICC, IF, IHC-Fr, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	KLH conjugated synthetic peptide derived from human Ki67
Conjugation:	Unconjugated
Alternative Names:	KIA, MIB-, MIB-1, PPP1R105, KI67_HUMAN, MKI67, Antigen identified by monoclonal antibody Ki-67 (Antigen KI-67 \| Antigen Ki67),

Ki-67 Rabbit Polyclonal Antibody

Clonality:	Polyclonal
Concentration:	1mg/ml
Molecular Weight:	320/359 kDa
Pubmed:	29173982
UniProt:	P46013
Buffer:	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:	Liquid
Target:	MKI67

Application Dilute:

WB=1:500-2000, IHC-P=1:200-800, IHC-F=1:200-800, ICC/IF=1:100-500, IF=1:200-800, Flow-Cyt=1ug/Test

	IHC-P of human oral squamous cell carcinoma using Ki67 antibody.
	4% Paraformaldehyde-fixed Hela (H) cell, Triton X-100 at r.t. for 20 min, Antibody incubation with (Ki-67) polyclonal Antibody, unconjugated (orb13523) 1:100, 90 min at 37C, followed by conjugated Goat Anti-Rabbit IgG antibody (green, orb868805) at 37C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
	Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (orb13523) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (Human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (orb13523) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human ovarian serous carcinoma, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (orb13523) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (orb13523) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

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