Lipoprotein lipase Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB13546
Article Name: Lipoprotein lipase Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB13546
Supplier Catalog Number: orb13546
Alternative Catalog Number: BYT-ORB13546-50,BYT-ORB13546-100,BYT-ORB13546-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human LPL (401-475/475aa)
Conjugation: Unconjugated
Alternative Names: HDLCQ11, LIPD, LIPL_BOVIN, LPL, Phospholipase A1, 3.1.1.34, LIPL_HUMAN, LIPL_MOUSE,
Lipoprotein lipase Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 52 kDa
Pubmed: 26336959
UniProt: P06858
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: LPL
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2µg /test
Immunohistochemical staining of rat spleen tissue using LPL antibody.
Immunohistochemical staining of rat small intestine tissue using LPL antibody.
Blank control (blue line): raji (fixed with pre-warmed 4% paraformaldehyde for 30 min at 37C and then permeabilized with 90% ice-cold methanol for 30 min on ice), Primary Antibody (green line): Rabbit Anti-Lipoprotein lipase antibody (orb13546), Dilution: 0.2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test.
Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Lipoprotein lipase) Polyclonal Antibody, Unconjugated (orb13546) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-Lipoprotein lipase (orb13546) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52kD, Observed band size: 55kD.
Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human MCF-7 cell lysates, Lane 3: Human HL-60 cell lysates, Primary: Anti-Lipoprotein lipase (orb13546) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kDa, Observed band size: 60 kDa.



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