Sm-D3/SNRPD3/D3 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB1474819
Article Name: Sm-D3/SNRPD3/D3 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB1474819
Supplier Catalog Number: orb1474819
Alternative Catalog Number: BYT-ORB1474819-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: E.coli-derived human Sm-D3/SNRPD3 recombinant protein (Position: M1-R112).
Conjugation: Unconjugated
Alternative Names: Mediator of RNA polymerase II transcription subunit 20, Mediator complex subunit 20, TRF-proximal protein homolog, hTRFP, MED20, TRFP
Anti-Sm-D3/SNRPD3 Antibody. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Clonality: Polyclonal
Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Molecular Weight: 16 kDa
UniProt: P62318
Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Form: Lyophilized
Target: Small nuclear ribonucleoprotein Sm D3
Application Dilute: Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse, Rat Immunofluorescence, 5 µg/ml, Human ELISA, 0.1-0.5 µg/ml, -
IF analysis of Sm-D3/SNRPD3 using anti-Sm-D3/SNRPD3 antibody. Sm-D3/SNRPD3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-Sm-D3/SNRPD3 Antibody overnight at 4C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of Sm-D3/SNRPD3 using anti-Sm-D3/SNRPD3 antibody. Sm-D3/SNRPD3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Sm-D3/SNRPD3 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of Sm-D3/SNRPD3 using anti-Sm-D3/SNRPD3 antibody. Sm-D3/SNRPD3 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Sm-D3/SNRPD3 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of Sm-D3/SNRPD3 using anti-Sm-D3/SNRPD3 antibody. Sm-D3/SNRPD3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Sm-D3/SNRPD3 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of Sm-D3/SNRPD3 using anti-Sm-D3/SNRPD3 antibody. Sm-D3/SNRPD3 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Sm-D3/SNRPD3 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of Sm-D3/SNRPD3 using anti-Sm-D3/SNRPD3 antibody. Sm-D3/SNRPD3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Sm-D3/SNRPD3 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of Sm-D3/SNRPD3 using anti-Sm-D3/SNRPD3 antibody. Sm-D3/SNRPD3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed