PTP1B Recombinant Rabbit Monoclonal Antibody, Clone: [R3F5], Unconjugated

Catalog Number: BYT-ORB1499282
Article Name: PTP1B Recombinant Rabbit Monoclonal Antibody, Clone: [R3F5], Unconjugated
Biozol Catalog Number: BYT-ORB1499282
Supplier Catalog Number: orb1499282
Alternative Catalog Number: BYT-ORB1499282-100,BYT-ORB1499282-25,BYT-ORB1499282-50
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, ICC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthesized peptide derived from human PTPN1 (350-400aa)
Conjugation: Unconjugated
Alternative Names: PTP1B, PTP-1B, PTP-HA2, Ptp, PTN1_HUMAN, PTPN1, Protein-tyrosine phosphatase 1B (PTP-1B), 3.1.3.48, PTN1_MOUSE, Protein-tyrosine phosphatase HA2 (PTP-HA2), PTN1_RAT,
PTP1B Recombinant Rabbit Monoclonal Antibody
Clonality: Recombinant
Concentration: 1mg/ml
Clone Designation: [R3F5]
Molecular Weight: 50 kDa
UniProt: P18031
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: PTPN1
Application Dilute: WB=1:500-2000, IHC-P=1:200-500, IHC-F=1:200-500, ICC/IF=1:50-200, IF=1:200-500, Flow-Cyt=1:50-100
25 ug total protein per lane of various lysates probed with PTP1B monoclonal antibody, unconjugated (orb1499282) at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated (orb1499282) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated (orb1499282) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated (orb1499282) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated (orb1499282) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated (orb1499282) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated (orb1499282) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated (orb1499282) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.