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Rabbit MafA Recombinant Monoclonal Antibody, Clone: [BLR067G], Unconjugated

Rabbit MafA Recombinant Monoclonal Antibody, Clone: [BLR067G], Unconjugated

Catalog Number: BYT-ORB1519835

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Article Name:	Rabbit MafA Recombinant Monoclonal Antibody, Clone: [BLR067G], Unconjugated
Biozol Catalog Number:	BYT-ORB1519835
Supplier Catalog Number:	orb1519835
Alternative Catalog Number:	BYT-ORB1519835-10,BYT-ORB1519835-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	ICC, IHC, WB
Species Reactivity:	Human, Mouse
Immunogen:	Between 125 and 175
Conjugation:	Unconjugated
Alternative Names:	hMafA, INSDM, pancreatic beta-cell-specific transcriptional activator, RIPE3b1, RIPE3b1 factor, transcription factor MafA, transcription factor RIPE3b1, v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A

MafA Antibody

Clonality:	Recombinant
Concentration:	1000 µg/ml
Clone Designation:	[BLR067G]
NCBI:	919331
UniProt:	Q8NHW3
Buffer:	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Form:	Liquid
Target:	MafA

Application Dilute:	WB - 1:1000, IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Zinc-fixative (JB fix) is recommended to enhance staining., ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recomm
Application Notes:	Application Notes: Format: Whole IgG

	Detection of mouse MafA by immunohistochemistry. Sample: FFPE section of mouseBeta-TC-6 cells. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519835). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of mouse MafA by immunohistochemistry. Sample: FFPE section of mouse pancreas (JB fixation). Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519835). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human MafA by immunohistochemistry. Sample: FFPE section of human pancreatic tumor. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519835). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human MafA (red) by immunohistochemistry. Sample: FFPE section of human pancreatic carcinoma. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519835) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal(TM). Counterstain: DAPI (blue).
	Detection of mouse MafA by western blot. Samples: Whole cell lysate (10 µg) from Beta-TC-6, AlphaTC1 Clone 9, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519835) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
	Detection of human MafA by western blot. Samples: Whole cell lysate (10 µg) from HEK293T transfected with myc tagged Human MafA, Human MafB, and Human cMaf prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519835) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit recombinant monoclonal antibody to Myc-tag.
	Detection of mouse MafA by western blot. Samples: Whole cell lysate (10 µg) from HEK293T transfected with myc tagged mouse MafA, Human MafB, and Human cMaf prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519835) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit recombinant monoclonal antibody to Myc-tag.

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