Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Purity:
Recombinant antibody was purified from cell culture supernatant
Form:
Liquid
Target:
PD-L1
Application Dilute:
Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 2 µl per 1 x 10 6 cells. ICC-IF 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope ret
Application Notes:
Application Notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight
Detection of human PD-L1 by western blot. Samples: Whole cell lysate (50 µg) from RKO, Jurkat, SUP-M2, HEK293T, and HDLM-2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody
Detection of human PD-L1 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG
Detection of human PD-L1 by immunocytochemistry. Sample: FFPE section of human SUP-M2 cells. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG
Detection of human PD-L1 (red) and Lamin-A/C (green) in FFPE lung carcinoma by IHC-IF. Antibody: Rabbit anti-PD-L1 recombinant monoclonal and rabbit anti-Lamin-A/C. Secondary: DyLight 594-conjugated goat anti-rabbit IgG and DyLight 488-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).
Detection of human PD-L1 (green) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal(TM). Counterstain: DAPI (blue).
Detection of human PD-L1 by immunocytochemistry. Sample: FFPE section of human SUP-M2 cells. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody Secondary: HRP-conjugated goat anti-rabbit IgG
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