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Rabbit CD45 Recombinant Monoclonal Antibody, Clone: [BL-178-12C7], Unconjugated

Rabbit CD45 Recombinant Monoclonal Antibody, Clone: [BL-178-12C7], Unconjugated

Catalog Number: BYT-ORB1519975

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Article Name:	Rabbit CD45 Recombinant Monoclonal Antibody, Clone: [BL-178-12C7], Unconjugated
Biozol Catalog Number:	BYT-ORB1519975
Supplier Catalog Number:	orb1519975
Alternative Catalog Number:	BYT-ORB1519975-10,BYT-ORB1519975-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	FC, ICC, IHC, IP, WB
Species Reactivity:	Human
Immunogen:	Between 1256 and C-terminus
Conjugation:	Unconjugated
Alternative Names:	B220, CD antigen CD45, CD45, CD45 antigen, CD45R, GP180, LCA, L-CA, Leukocyte common antigen, LY5, protein tyrosine phosphatase, receptor type, c polypeptide, receptor-type tyrosine-protein phosphatase C, T200, T200 glycoprotein, T200 leukocyte common antigen

CD45 Antibody

Clonality:	Recombinant
Concentration:	50 µg/ml
Clone Designation:	[BL-178-12C7]
NCBI:	563578
UniProt:	P08575
Buffer:	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Form:	Liquid
Target:	CD45

Application Dilute:	WB - 1:1000, IP - 20 µl/mg lysate, IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections., IHC-IF
Application Notes:	Application Notes: Format: Whole IgG

	Detection of human CD45 by immunocytochemistry. Sample: FFPE section of Daudi cells. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519975). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human CD45 by immunocytochemistry. Sample: FFPE section of Jurkat cells. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519975). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human CD45 by immunohistochemistry. Sample: FFPE section of appendix. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519975). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human CD45 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519975). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human CD45 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519975) used for IP at 20 µl/mg lysate. CD45 was also immunoprecipitated by a previous lot of this antibody (orb1519975). For blotting immunoprecipitated CD45, orb1519975 was used at 1:1000. Chemiluminescence with an exposure time of 1 second.
	Detection of human CD45 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, MOLT-4, HeLa, and SR cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519975) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

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