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Rabbit 53BP1 Recombinant Monoclonal Antibody, Clone: [BL-250-1H11], Unconjugated

Rabbit 53BP1 Recombinant Monoclonal Antibody, Clone: [BL-250-1H11], Unconjugated

Catalog Number: BYT-ORB1519978

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Article Name:	Rabbit 53BP1 Recombinant Monoclonal Antibody, Clone: [BL-250-1H11], Unconjugated
Biozol Catalog Number:	BYT-ORB1519978
Supplier Catalog Number:	orb1519978
Alternative Catalog Number:	BYT-ORB1519978-100,BYT-ORB1519978-20
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	FC, IHC, IP, WB
Species Reactivity:	Human, Mouse
Immunogen:	Between 350 and 400
Conjugation:	Unconjugated
Alternative Names:	53BP1, p202, p53-binding protein 1, p53BP1, TDRD30, TP53-binding protein 1, tumor protein 53-binding protein, 1, tumor suppressor p53-binding protein 1

53BP1 Antibody

Clonality:	Recombinant
Concentration:	100 µg/ml
Clone Designation:	[BL-250-1H11]
NCBI:	005648
UniProt:	Q12888
Buffer:	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Form:	Liquid
Target:	53BP1

Application Dilute:	WB - 1:1000, IP - 20 µl/mg lysate, IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Application Notes:	Application Notes: Format: Whole IgG

	Detection of human 53BP1 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
	Detection of mouse 53BP1 (shaded) in mIMCD-3 cells by flow cytometry. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
	Detection of human 53BP1 by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
	Detection of mouse 53BP1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
	Detection of human 53BP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) used for IP at 20 µl per reaction.
	Detection of human 53BP1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK) , and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (orb1519978) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 second.

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