Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Form:
Liquid
Target:
COPB2
Application Dilute:
WB - 1:10,000 - 1:25,000, IP - 2 - 10 µg/mg lysate, IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Application Notes:
Application Notes: Format: Whole IgG
Detection of human COPB2 by immunohistochemistry. Samples: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-COPB2 used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB
Detection of mouse COPB2 by immunohistochemistry. Samples: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-COPB2 used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of human COPB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COPB2 antibody (orb1522847) used for IP at 6 µg per reaction. COPB2 was also immunoprecipitated by a previous lot of this antibody (orb1522847) and a second antibody against a different epitope of COPB2.
Detection of mouse COPB2 by western blot. Samples: Whole cell lysate (5 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COPB2 antibody (orb1522847) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Detection of human COPB2 by western blot. Samples: Whole cell lysate (5 µg) from HEK293T, HeLa, U2OS, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COPB2 antibody (orb1522847) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
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