Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Form:
Liquid
Target:
ICLN
Application Dilute:
WB - 1:2,000 - 1:10,000, IP - 2 - 10 µg/mg lysate, IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Application Notes:
Application Notes: Format: Whole IgG
Detection of human ICLN (shaded) in A549 cells by flow cytometry.Antibody: Rabbit anti-ICLN polyclonal antibody (orb1522851) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
Detection of mouse ICLN (shaded) in A20 cells by flow cytometry.Antibody: Rabbit anti-ICLN polyclonal antibody (orb1522851) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
Detection of human ICLN by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ICLN (Cat. No. orb1522851) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of mouse ICLN by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-ICLN (Cat. No. orb1522851) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of human ICLN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction, 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ICLN antibody orb1522851 used for IP at 6 µg per reaction.
Detection of human and mouse ICLN by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ICLN antibody orb1522851 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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