Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Form:
Liquid
Target:
IDH2
Application Dilute:
WB - 1:1,000 - 1:5,000, IP - 2 - 10 µg/mg lysate, IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC-IF - 1:500 - 1:2,500. Formaldehyde fixation is recommended. Permeabilization with Triton-X
Application Notes:
Application Notes: Format: Whole IgG
Detection of human IDH2 by immunocytochemistry.Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-IDH2 (Cat. No. orb1523620) used at a dilution of 1: 1, 000 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1: 100.
Detection of mouse IDH2 by immunohistochemistry. Samples: FFPE sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-IDH2 (Cat. No. orb1523620) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of human IDH2 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma (left) and lung cancer (right). Antibody: Affinity purified rabbit anti-IDH2 (Cat. No. orb1523620) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of human IDH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-IDH2 antibody orb1523620 used for IP at 6 µg/mg lysate.
Detection of human and mouse IDH2 by western blot. Samples: Whole cell lysate (50 µg) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-IDH2 antibody orb1523620 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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