Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Form:
Liquid
Target:
Sp1
Application Dilute:
WB - 1:2,000 - 1:10,000, IP - 2 - 10 µg/mg lysate, IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Application Notes:
Application Notes: Format: Whole IgG
Detection of human Sp1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified goat anti-Sp1 (Cat. No. orb1523885) used at a dilution of 1: 5, 000 (0.2 µg/ml). Detection: DAB
Detection of mouse Sp1 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified goat anti-Sp1 (Cat. No. orb1523885) used at a dilution of 1: 1, 000 (1 µg/ml). Detection: DAB
Detection of human Sp1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified goat anti-Sp1 antibody orb1523885 used for IP at 6 µg/mg lysate.
Detection of mouse Sp1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, Renca, and TCMK-1 cells. Antibodies: Affinity purified goat anti-Sp1 antibody orb1523885 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human Sp1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (50µg) , and Jurkat (50µg) cells. Antibodies: Affinity purified goat anti-Sp1 antibody orb1523885 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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