Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Form:
Liquid
Target:
hnRNP-L
Application Dilute:
WB - 1:2,000 - 1:10,000, IP - 2 - 10 µg/mg lysate, IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Application Notes:
Application Notes: Format: Whole IgG
Detection of human hnRNP-L by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-hnRNP-L (Cat. No. orb1523975) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB
Detection of mouse hnRNP-L by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-hnRNP-L (Cat. No. orb1523975) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of human hnRNP-L by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-hnRNP-L antibody orb1523975 used for IP at 6 µg/mg lysate.
Detection of human hnRNP-L by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (50µg) , and Jurkat (50µg) cells. Antibodies: Affinity purified rabbit anti-hnRNP-L antibody orb1523975 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of mouse hnRNP-L by western blot. Samples: Whole cell lysate (15 and 50 µg) from mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-hnRNP-L antibody orb1523975 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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