Rabbit CD44 Antibody, Unconjugated, Polyclonal
Catalog Number:
BYT-ORB1524021
- Images (5)
| Article Name: | Rabbit CD44 Antibody, Unconjugated, Polyclonal |
| Biozol Catalog Number: | BYT-ORB1524021 |
| Supplier Catalog Number: | orb1524021 |
| Alternative Catalog Number: | BYT-ORB1524021-10, BYT-ORB1524021-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IHC, IP, WB |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Between 692 and C-terminus |
| Conjugation: | Unconjugated |
| Alternative Names: | CD antigen CD44, CD44 antigen, CDW44, cell surface glycoprotein CD44, chondroitin sulfate proteoglycan 8, CSPG8, ECMR-III, epican, extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HCELL, hematopoietic cell E- and L-selectin ligand, heparan sulfate proteoglycan, Hermes antigen, homing function and Indian blood group system, HUTCH-I, hyaluronate receptor, IN, Indian blood group antigen, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, PGP-1, PGP-I, phagocytic glycoprotein 1, Phagocytic glycoprotein I, soluble CD44 |
| CD44 Antibody |
| Application Dilute: | WB - 1:2,000 - 1:10,000, IP - 2 - 10 µg/mg lysate, IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Application Notes: | Application Notes: Format: Whole IgG |
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Detection of human CD44 by immunohistochemistry.Sample: FFPE section of human head and neck squamous cell carcinoma.Antibody: Affinity purified rabbit anti-CD44 (Cat. No. orb1524021) used at a dilution of 1: 5, 000 (0.2µg/ml). Substrate: DAB |
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Detection of human CD44 by immunohistochemistry.Sample: FFPE section of human melanoma.Antibody: Affinity purified rabbit anti-CD44 (Cat. No. orb1524021) used at a dilution of 1: 5, 000 (0.2µg/ml). Substrate: DAB |
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Detection of human CD44 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg for IP, 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CD44 antibody orb1524021 used for IP at 2 µg per reaction. |
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Detection of mouse CD44 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD44 antibody orb1524021 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. |
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Detection of human CD44 by western blot. Samples: Whole cell lysate (10 µg) from A-549, HEK293T, HeLa, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD44 antibody orb1524021 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds. |
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