Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Form:
Liquid
Target:
PCNA
Application Dilute:
WB - 1:5,000 - 1:20,000, IP - 2 - 10 µg/mg lysate, IHC - 1:2,000 to 1:10,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections., IHC-IF - 1:1,000 - 1:5,000
Application Notes:
Application Notes: Format: Whole IgG
Detection of mouse PCNA by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-PCNA used at a dilution of 1:5000 (0.2 µg/ml).
Detection of human PCNA by immunohistochemistry. Sample: FFPE section of human breast. Antibody: Affinity purified rabbit anti-PCNA used at a dilution of 1:5000 (0.2 µg/ml).
Detection of human PCNA by IHC. Sample: human stomach carcinoma. Antibody: Affinity purified rabbit anti-PCNA at 1:4, 000. Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated.
Detection of human PCNA by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-PCNA antibody.
Detection of mouse PCNA by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-PCNA antibody at 0.1 µg/ml.
Detection of human PCNA by western blot.Samples: Whole cell lysate from A-549, Jurkat, HEK293T, MCF-7, and HeLa cells. Antibody: Affinity purified rabbit anti-PCNA antibody at 0.1 µg/ml.
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