Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Form:
Liquid
Target:
RbBP5
Application Dilute:
WB - 1:10,000 - 1:25,000, IP - 2 - 10 µg/mg lysate, IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections., ICC - 1:250 - 1:1,000, ChIP - 1 - 3 µg as per Dou et al., Nat Struct Mol Biol 13 (8):713-719
Application Notes:
Application Notes: Format: Whole IgG
ChIP-chip scatter plot of anti-RbBP5 enriched DNA binding sites versus input reference DNA.
Localization of RbBP5 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-RbBP5 antibody and analyzed by DNA sequencing.
Detection of human RbBP5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RbBP5 used at a dilution of 1:1, 000 (1µg/ml).
Detection of human RbBP5 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-RbBP5 antibody.
Detection of human RbBP5 by western blot.Samples: Whole cell lysate from HeLa, NCI-H460, HEK293T, Malme-3M, and Jurkat cells. Antibody: Affinity purified rabbit anti-RbBP5 antibody at 0.04 µg/ml.
Detection of mouse RbBP5 by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-RbBP5 antibody at 0.04 µg/ml.
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