C22orf43 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB156020
Article Name: C22orf43 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB156020
Supplier Catalog Number: orb156020
Alternative Catalog Number: BYT-ORB156020-50,BYT-ORB156020-100,BYT-ORB156020-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Rat
Immunogen: KLH conjugated synthetic peptide derived from human C22orf43 (1-100/229aa)
Conjugation: Unconjugated
Alternative Names: C22orf43, KB-208E9.1, DRIC1_HUMAN, DRICH1,
C22orf43 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 25 kDa
Pubmed: 30970261
UniProt: Q6PGQ1
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: DRICH1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Western blot analysis of U251 human Cell Lysate using C22orf43 antibody.
Western blot analysis of Human HT29 Cell Lysate using C22orf43 antibody.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (C22orf43) Polyclonal Antibody, Unconjugated (orb156020) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (C22orf43) Polyclonal Antibody, Unconjugated (orb156020) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: HT29 (huaman) Cell Lysate at 40 ug, Primary: Anti-C22orf43 (orb156020) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25kD, Observed band size: 23kD.
Sample: U251 (human) Cell Lysate at 40 ug, Primary: Anti-C22orf43 (orb156020) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25kD, Observed band size: 22kD.



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