C3orf32 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB156052
Article Name: C3orf32 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB156052
Supplier Catalog Number: orb156052
Alternative Catalog Number: BYT-ORB156052-50,BYT-ORB156052-100,BYT-ORB156052-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human C3orf32 (51-150/353aa)
Conjugation: Unconjugated
Alternative Names: C3orf32, SSU-2, fls485, SSUH2_HUMAN, SSUH2, Protein ssu-2 homolog,
C3orf32 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 40 kDa
UniProt: Q9Y2M2
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: SSUH2
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of rat testis tissue using C3orf32 antibody.
Immunohistochemical staining of rat small intestine tissue using C3orf32 antibody.
Paraformaldehyde-fixed, paraffin embedded (Mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (C3orf32) Polyclonal Antibody, Unconjugated (orb156052) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (C3orf32) Polyclonal Antibody, Unconjugated (orb156052) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (chromosome 3 open reading frame 32, C3orf32) Polyclonal Antibody, Unconjugated (orb156052) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Lane 1: Testis (Mouse) Lysate at 40 ug, Lane 2: Epididymis (Rat) Lysate at 40 ug, Lane 3: Small intestine (Rat) Lysate at 40 ug, Primary: Anti-C3orf32 (orb156052) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kD, Observed band size: 40 kD.



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