C4orf52 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB156075
- Images (8)
| Article Name: | C4orf52 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB156075 |
| Supplier Catalog Number: | orb156075 |
| Alternative Catalog Number: | BYT-ORB156075-50,BYT-ORB156075-100,BYT-ORB156075-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human C4orf52 (101-168/168aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | C4orf52, MITRAC7, PNX, SIM20_HUMAN, SMIM20, Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 7 kDa (MITRAC7), |
| C4orf52 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 7.7/18 kDa |
| UniProt: | Q8N5G0 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | SMIM20 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Western blot analysis of mouse Heart lysates (Lane 2) using C4orf52 antibody. |
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Western blot analysis of Mouse Intestine Lysate using C4orf52 antibody. |
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Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (C4orf52) Polyclonal Antibody, Unconjugated (orb156075) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (C4orf52) Polyclonal Antibody, Unconjugated (orb156075) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (C4orf52) Polyclonal Antibody, Unconjugated (orb156075) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Sample: heart (Mouse) Lysate at 40 ug, Primary: Anti-C4orf52 (orb156075) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 18 kD, Observed band size: 18 kD. |
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Sample: muscle (Mouse) Lysate at 40 ug, Primary: Anti-C4orf52 (orb156075) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 18 kD, Observed band size: 18 kD. |
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Tissue/Cell: Rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-C4orf52 Polyclonal Antibody, Unconjugated (orb156075) 1:5000, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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