Phospho-ITGB2 (Thr758) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB156299
Article Name: Phospho-ITGB2 (Thr758) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB156299
Supplier Catalog Number: orb156299
Alternative Catalog Number: BYT-ORB156299-50,BYT-ORB156299-100,BYT-ORB156299-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC-Fr, IHC-P
Species Reactivity: Human
Immunogen: KLH conjugated synthesised phosphopeptide derived from human CD18 around the phosphorylation site of Thr758 SA(p-T)TT
Conjugation: Unconjugated
Alternative Names: ITGB2 | CD18 (p-T758), p-CD18, phospho-CD18, CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7, ITB2_HUMAN, ITGB2, Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, Complement receptor C3 subunit beta,
Phospho-ITGB2 (Thr758) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 82 kDa
UniProt: P05107
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: ITGB2
Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
Application Notes: Modification: Phosphorylated
Immunohistochemical staining of human lung carcinoma tissue using CD18 (Phospho-Thr758) antibody.
Immunohistochemical staining of human kidney tissue using CD18 (Phospho-Thr758) antibody.
Blank control: Raji cells (blue). Primary Antibody: Rabbit Anti-Phospho-CD18 (Thr758) antibody (orb156299), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.
Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-CD18 (Thr758) Polyclonal Antibody, Unconjugated (orb156299) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-CD18 (Thr758) Polyclonal Antibody, Unconjugated (orb156299) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-CD18 (Thr758) Polyclonal Antibody, Unconjugated (orb156299) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



* VAT and and shipping costs not included.
Errors and price changes excepted
Availability
ORDER BY EMAIL
Product Guarantee and Expert Support
BIOZOL