Phospho-c-Fos (Ser362) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB156346
Article Name: Phospho-c-Fos (Ser362) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB156346
Supplier Catalog Number: orb156346
Alternative Catalog Number: BYT-ORB156346-50,BYT-ORB156346-100,BYT-ORB156346-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated Synthesised phosphopeptide derived from human c-Fos around the phosphorylation site of Ser362 KG(p-S)SS
Conjugation: Unconjugated
Alternative Names: FOS | c-Fos (p-S362), p-c-Fos, phospho-c-Fos, AP-1, C-FOS, p55, D12Rfj1, cFos, FOS_HUMAN, FOS, Cellular oncogene fos, Fos proto-oncogene, AP-1 transcription factor subunit, G0/G1 switch regulatory protein 7, Proto-oncogene c-Fos, Transcription factor AP-1 subunit c-Fos, G0S7, FOS_MOUSE, FBJ osteosarcoma oncogene, FOS_RAT,
Phospho-c-Fos (Ser362) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 41 kDa
UniProt: P01100
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: FOS
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Application Notes: Modification: Phosphorylated
Immunohistochemical staining of mouse osteosarcoma tissue using c-Fos (Phospho-Ser362) antibody.
Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse osteosarcoma labeled with Anti-Phospho-c-Fos (Ser362) Polyclonal Antibody, Unconjugated (orb156346) at 1:100 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-c-Fos (Ser362)) Polyclonal Antibody, Unconjugated (orb156346) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-c-Fos (Ser362)) Polyclonal Antibody, Unconjugated (orb156346) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, A431 (Human) Cell Lysate at 30 ug, Adrenal glands (Mouse) Lysate at 40 ug, Adrenal glands (Rat) Lysate at 40 ug, Uterus (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-c-Fos (Ser362) (orb156346) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62/46 kD, Observed band size: 46 kD.
Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-c-Fos (Ser362) (orb156346) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41 kD.



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