Phospho-CK18 (Ser52) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB156372
- Images (8)
| Article Name: | Phospho-CK18 (Ser52) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB156372 |
| Supplier Catalog Number: | orb156372 |
| Alternative Catalog Number: | BYT-ORB156372-50,BYT-ORB156372-100,BYT-ORB156372-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Mouse, Rat |
| Immunogen: | KLH conjugated synthesised phosphopeptide derived from mouse CK18 around the phosphorylation site of Ser52 SR(p-S)VW |
| Conjugation: | Unconjugated |
| Alternative Names: | KRT18 | CK-18 (p-S52), p-CK-18, phospho-CK-18, CK-18, CYK18, K18, CK18, Krt1-18, K1C18_HUMAN, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18 (CK-18), Keratin-18 (K18), K1C18_MOUSE, Cytokeratin endo B (Keratin D), Kerd, K1C18_RAT, |
| Phospho-CK18 (Ser52) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 48 kDa |
| UniProt: | P05784 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | KRT18 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Application Notes: | Modification: Phosphorylated |
|
|
Paraformaldehyde-fixed, paraffin embedded (Mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-CK18 (Ser52)) Polyclonal Antibody, Unconjugated (orb156372) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-CK18 (Ser52)) Polyclonal Antibody, Unconjugated (orb156372) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (p-CK18 (Ser52)) Polyclonal Antibody, Unconjugated (orb156372) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (Rat skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-CK18 (Ser52)) Polyclonal Antibody, Unconjugated (orb156372) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (Rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (p-CK18 (Ser52)) Polyclonal Antibody, Unconjugated (orb156372) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
|
|
Sample: Lane 1: Pancreas (Mouse) Lysate at 40 ug, Lane 2: Liver (Mouse) Lysate at 40 ug, Lane 3: Lung (Mouse) Lysate at 40 ug, Primary: Anti-phospho-CK18 (SER52) (orb156372) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 48 kD. |
|
|
IHC-P analysis of Rat skin using phospho-CK18Ser52 Polyclonal Antibody at 1:400 dilution. |
|
|
IHC-P analysis of Mouse placenta using phospho-CK18 Ser52 Polyclonal Antibody at 1:400 dilution. |
Product Guarantee and Expert Support
