0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% ProClin300 and 50% Glycerol
Form:
Liquid
Target:
CTLA4
Application Dilute:
WB=1:500-2000, Flow-Cyt=1µg/Test
Western blot analysis of k562 lysates using CTLA4 antibody
Blank control (blue): Molt-4 Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CTLA4/FITC Conjugated antibody, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/FITC orange), used under the same conditions.
Blank control (blue): Mouse Spleen Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Phospho-c-Fos (Thr325)/FITC Conjugated antibody, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/FITC (orange), used under the same conditions.
Blank control: Hela (blue), the cells were fixed with 2% paraformaldehyde (10 min), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA (green).
Blank control: Raji (blue). Primary Antibody: Rabbit Anti-CTLA4 antibody (orb156486), Dilution: 5 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.
Sample: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-CTLA4 (orb156486) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 35 kD.
Sample: Liver (Mouse) Cell Lysate at 30 ug, Primary: Anti-CTLA4 (orb156486) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 35 kD.
Sample: Liver (Rat) Cell Lysate at 30 ug, Primary: Anti-CTLA4 (orb156486) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 35 kD.
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