EEF1G Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB156700
Article Name: EEF1G Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB156700
Supplier Catalog Number: orb156700
Alternative Catalog Number: BYT-ORB156700-50,BYT-ORB156700-100,BYT-ORB156700-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human EEF1G (251-350/437aa)
Conjugation: Unconjugated
Alternative Names: EF1G, GIG35, EF1G_HUMAN, EEF1G, EF-1-gamma, eEF-1B gamma,
EEF1G Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 50 kDa
UniProt: P26641
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: EEF1G
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Western blot analysis of mouse Cerebrum lysates using EEF1G antibody.
Western blot analysis of human Hela lysates using EEF1G antibody.
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (EEF1G) Polyclonal Antibody, Unconjugated (orb156700) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (EEF1G) Polyclonal Antibody, Unconjugated (orb156700) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-EEF1G (orb156700) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.
Sample: Hela Cell (Human) Lysate at 40 ug, Primary: Anti-EEF1G (orb156700) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.



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