Human IgG CY5 conjugated, Cy5 isotype control for flow cytometry
HUMAN IgG whole molecule CY5 Antibody
Concentration:
1.0 mg/mL
Buffer:
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free, Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Human IgG whole molecule CY5 conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above. Human IgG whole molecule CY5 conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human IgG and anti-Human Serum.
Form:
Lyophilized
Application Dilute:
FC: User Optimized, IHC: User Optimized, WB: User Optimized
Application Notes:
Application Notes: Human IgG whole molecule CY5 conjugated has been tested in dot blot and can be utilized as a control reagent in Flow Cytometry, Immunohistochemistry, and Western Blotting. Researchers should determine optimal titers for applications that are not stated
Dot Blot Results of Human IgG Whole Molecule Cy5 Conjugated. Membrane: 0.45 µm LF-PVDF. Blotted: Human IgG Whole Molecule Cy5 Conjugated at 3-fold dilution: 100 ng, 33.33 ng, 11.11 ng, 3.7 ng, 1.23 ng. Blocked: Universal Blocking Buffer (p/n orb348644) for 30 min at RT.
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