FKBP10 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB156888
Article Name: FKBP10 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB156888
Supplier Catalog Number: orb156888
Alternative Catalog Number: BYT-ORB156888-50,BYT-ORB156888-100,BYT-ORB156888-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human FKBP10 (31-130/582aa)
Conjugation: Unconjugated
Alternative Names: BRKS, BRKS1, FKBP65, OI11, OI6, PPIASE, TLH1, hFKBP65, FKB10_HUMAN, FKBP10, PPIase FKBP10, 65 kDa FK506-binding protein (65 kDa FKBP | FKBP-65), FK506-binding protein 10 (FKBP-10), Immunophilin FKBP65, Rotamase, 5.2.1.8,
FKBP10 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 61 kDa
UniProt: Q96AY3
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: FKBP10
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of rat brain tissue using FKBP10 antibody.
Western blot analysis of extracts from MCF-7 cell using FKBP10 antibody.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (FKBP10) Polyclonal Antibody, Unconjugated (orb156888) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (FKBP10) Polyclonal Antibody, Unconjugated (orb156888) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human LO2 cell lysates, Primary: Anti-FKBP10 (orb156888) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 61 kDa, Observed band size: 77 kDa.
Sample: Uterus (Mouse) Lysate at 40 ug, Primary: Anti-FKBP10 (orb156888) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 61 kD, Observed band size: 76 kD.



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