Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GABRB2) Polyclonal Antibody, Unconjugated (orb157008) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GABRB2) Polyclonal Antibody, Unconjugated (orb157008) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Cerebellum (rat) Lysate at 40 ug, Cerebellum (mouse) Lysate at 40 ug, Primary: Anti-GABRB2 (orb157008) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56kD, Observed band size: 56 kD.
Sample: Lane 1: Mouse Cerebellum tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Rat Cerebellum tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Primary: Anti-GABRB2 (orb157008) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kDa, Observed band size: 50 kDa.
IHC-P analysis of Mouse brain using GABRB2 Polyclonal Antibody at 1:400 dilution.
IHC-P analysis of rat brain using GABRB2 Polyclonal Antibody at 1:200 dilution.
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