QARS/Glutamyl Prolyl tRNA synthetase/ProRS Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB157190
Article Name: QARS/Glutamyl Prolyl tRNA synthetase/ProRS Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB157190
Supplier Catalog Number: orb157190
Alternative Catalog Number: BYT-ORB157190-50,BYT-ORB157190-100,BYT-ORB157190-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human ProRS (651-750/1512aa)
Conjugation: Unconjugated
Alternative Names: GLNRS, MSCCA, PRO2195, QARS, SYQ_HUMAN, QARS1, Glutaminyl-tRNA synthetase (GlnRS), 6.1.1.18,
QARS/Glutamyl Prolyl tRNA synthetase/ProRS Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 171 kDa
UniProt: P47897
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: QARS1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of Rat stomach tissue using Glutamyl Prolyl tRNA synthetase antibody.
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (QARS Glutamyl Prolyl tRNA synthetase) Polyclonal Antibody, Unconjugated (orb157190) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (QARS Glutamyl Prolyl tRNA synthetase) Polyclonal Antibody, Unconjugated (orb157190) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (QARS Glutamyl Prolyl tRNA synthetase) Polyclonal Antibody, Unconjugated (orb157190) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-QARS (orb157190) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 171 kD, Observed band size: 171 kD.
Tissue/Cell: Rat stomach tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Glutamyl Prolyl tRNA synthetase/ProRS Polyclonal Antibody, Unconjugated (orb157190) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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