Phospho-HSP70 (Tyr41) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB157589
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| Article Name: | Phospho-HSP70 (Tyr41) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB157589 |
| Supplier Catalog Number: | orb157589 |
| Alternative Catalog Number: | BYT-ORB157589-50,BYT-ORB157589-100,BYT-ORB157589-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human HSP70 around the phosphorylation site of Tyr41 PS(p-Y)VA |
| Conjugation: | Unconjugated |
| Alternative Names: | HSPA1A | HSP70 (p-Y41), p-HSP70, phospho-HSP70, HSP70-1, HSPA1, HEL-S-103, HSP70, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, Hsp70-3, Hsp70.3, hsp68, hsp70A1, Hspa1a, Hspa2, HS71A_BOVIN, Heat shock 70 kDa protein 1 (HSP70.1), HS71A_HUMAN, Heat shock 70 kDa protein 1 (HSP70-1 | HSP70.1), Heat shock protein family A member 1A, HSX70, HS71B_HUMAN, HSPA1B, Heat shock 70 kDa protein 2 (HSP70-2 | HSP70.2), Heat shock protein family A member 1B, HS71B_MOUSE, Hcp70.1, HS71A_MOUSE, Heat shock 70 kDa protein 3 (HSP70.3), HSP71_RAT, |
| Phospho-HSP70 (Tyr41) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 70 kDa |
| UniProt: | P0DMV8 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | HSPA1A |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test |
| Application Notes: | Modification: Phosphorylated |
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Flow cytometric analysis of Jurkat cells using HSP70 (Phospho-Tyr41) antibody. |
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Immunohistochemical staining of Rat pancreas tissue using HSP70 (Phospho-Tyr41) antibody. |
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Blank control (blue line): Jurkat (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-phospho-HSP70 (Tyr41) antibody (orb157589), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. |
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Blank control: A549. Primary Antibody (green line): Rabbit Anti-phospho-HSP70 (Tyr41) antibody (orb157589), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-phospho-HSP70 (Tyr41) antibody (orb157589), Dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Sample: A549 (Human) Cell Lysate at 30 ug, K562 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-HSP70 (Tyr41) (orb157589) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 70 kD, Observed band size: 70 kD. |
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Sample: Epithelium (Mouse) Lysate at 40 ug, Primary: Anti-phospho-HSP70 (Tyr41) (orb157589) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 70 kD, Observed band size: 70 kD. |
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Tissue/Cell: Rat pancreas tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-HSP70 (Tyr41) polyclonal Antibody, Unconjugated (orb157589) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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