HSP70 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB157591
Article Name: HSP70 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB157591
Supplier Catalog Number: orb157591
Alternative Catalog Number: BYT-ORB157591-50,BYT-ORB157591-100,BYT-ORB157591-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, ICC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human HSP70 (500-600/641aa)
Conjugation: Unconjugated
Alternative Names: HSP70-1, HSPA1, HEL-S-103, HSP70, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, Hsp70-3, Hsp70.3, hsp68, hsp70A1, Hspa1a, Hspa2, HS71A_BOVIN, Heat shock 70 kDa protein 1 (HSP70.1), HS71A_HUMAN, Heat shock 70 kDa protein 1 (HSP70-1 | HSP70.1), Heat shock protein family A member 1A, HSX70, HS71B_HUMAN, HSPA1B, Heat shock 70 kDa protein 2 (HSP70-2 | HSP70.2), Heat shock protein family A member 1B, HS71B_MOUSE, Hcp70.1, HS71A_MOUSE, Heat shock 70 kDa protein 3 (HSP70.3), HSP71_RAT,
HSP70 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 70 kDa
Pubmed: 41316514
UniProt: P0DMV8
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: HSPA1A
Application Dilute: WB=1:1000-5000, IHC-P=1:200-1000, IHC-F=1:200-1000, ICC/IF=1:100-500, IF=1:200-1000, Flow-Cyt=1ug/Test
Blank control: 293T. Primary Antibody (green line): Rabbit Anti-HSP70 antibody (orb157591), Dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control: A549. Primary Antibody (green line): Rabbit Anti-HSP70 antibody (orb157591), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control: A549. Primary Antibody (green line): Rabbit Anti-HSP70 antibody (orb157591), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSP70) Polyclonal Antibody, Unconjugated (orb157591) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSP70) Polyclonal Antibody, Unconjugated (orb157591) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (HSP70) Polyclonal Antibody, Unconjugated (orb157591) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Testis (Mouse) Lysate at 40 ug, Lane 2: Epididymis (Mouse) Lysate at 40 ug, Lane 3: Seminal vesicle (Mouse) Lysate at 40 ug, Lane 4: Liver (Mouse) Lysate at 40 ug, Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 6: Testis (Rat) Lysate at 40 ug, Lane 7: Spleen (Rat) Lysate at 40 ug, Lane 8: Hela (Human) Cell Lysate at 30 ug, Lane 9: Jurkat (Human) Cell Lysate at 30 ug, Lane 10: HepG2 (Human) Cell Lysate at 30 ug, Lane 11: A549 (Human) Cell Lysate at 30 ug, Lane 12: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-HSP70 (orb157591) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dil



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