PDE7A Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB158126
- Images (8)
| Article Name: | PDE7A Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB158126 |
| Supplier Catalog Number: | orb158126 |
| Alternative Catalog Number: | BYT-ORB158126-50,BYT-ORB158126-100,BYT-ORB158126-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human PDE7A (341-440/482aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | HCP1, PDE7, PDE7A_HUMAN, PDE7A, TM22, cAMP-specific phosphodiesterase 7A, 3.1.4.53, |
| PDE7A Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 55 kDa |
| UniProt: | Q13946 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | PDE7A |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test |
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Blank control (blue): RSC96 cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-PDE7A antibody (orb158126), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA. |
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Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PDE7A) Polyclonal Antibody, Unconjugated (orb158126) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Human Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PDE7A Polyclonal Antibody, Unconjugated (orb158126) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PDE7A Polyclonal Antibody, Unconjugated (orb158126) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Sample: Hela Cell (Human) Lysate at 30 ug, Primary: Anti-PDE7A (orb158126) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55kD, Observed band size: 55kD. |
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Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-PDE7A (orb158126) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 55kD, Observed band size: 55kD. |
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Flow cytometric analysis of Rsc96 cell using PDE7A antibody. |
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Western blot analysis of Mouse Muscle Lysate using PDE7A antibody. |
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