TJP2 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB158612
Article Name: TJP2 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB158612
Supplier Catalog Number: orb158612
Alternative Catalog Number: BYT-ORB158612-50,BYT-ORB158612-100,BYT-ORB158612-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human TJP2 (551-650/1190aa)
Conjugation: Unconjugated
Alternative Names: C9DUPq21.11, DFNA51, DUP9q21.11, FHCA1, PFIC4, X104, ZO2, ZO-2, ZO2_HUMAN, TJP2, Tight junction protein ZO-2, Zona occludens protein 2, Zonula occludens protein 2, ZO2_MOUSE,
TJP2 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 131 kDa
UniProt: Q9UDY2
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: TJP2
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TJP2) Polyclonal Antibody, Unconjugated (orb158612) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-TJP2 (orb158612) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 131 kD, Observed band size: 170 kD.
Tissue/Cell: Human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-TJP2 Polyclonal Antibody, Unconjugated (orb158612) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: Rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-TJP2 Polyclonal Antibody, Unconjugated (orb158612) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical staining of rat brain tissue using TJP2 antibody.
Immunohistochemical staining of Human kidney tissue using TJP2 antibody.



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