TMEM176A Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB158621
Article Name: TMEM176A Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB158621
Supplier Catalog Number: orb158621
Alternative Catalog Number: BYT-ORB158621-50,BYT-ORB158621-100,BYT-ORB158621-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse
Immunogen: KLH conjugated synthetic peptide derived from MOUSE TMEM176A (1-100/235aa)
Conjugation: Unconjugated
Alternative Names: GS188, HCA112, MS4B1, 0610011I04Rik, Keg2, RGD1310725, T176A_HUMAN, TMEM176A, Hepatocellular carcinoma-associated antigen 112, T176A_MOUSE, Gene signature 188, Kidney-expressed gene 2 protein, T176A_RAT,
TMEM176A Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 26 kDa
UniProt: Q9DCS1
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: TMEM176A
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TMEM176A) Polyclonal Antibody, Unconjugated (orb158621) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Protein: HepG2 (human) lysates at 30 ug, Primary: rabbit Anti-TMEM176A (orb158621) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 26 kD, Observed band size: 26 kD.
Protein: mouse kidney lysates, 30 ug, Primary: Anti-TMEM176A (orb158621) at 1:300, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:5000, ECL excitated the fluorescence, Predicted band size: 24kD, Observed band size: 24kD.
Sample: U937 (Human) Cell Lysate at 30 ug, Primary: Anti-TMEM176A (orb158621) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 26 kD, Observed band size: 25 kD.
Tissue/Cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-TMEM176A Polyclonal Antibody, Unconjugated (orb158621) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Western blot analysis of HepG2 lysates using TMEM176A antibody



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