STIP1 Recombinant Rabbit Monoclonal Antibody, Clone: [B5C7], Unconjugated

Catalog Number: BYT-ORB1592789
Article Name: STIP1 Recombinant Rabbit Monoclonal Antibody, Clone: [B5C7], Unconjugated
Biozol Catalog Number: BYT-ORB1592789
Supplier Catalog Number: orb1592789
Alternative Catalog Number: BYT-ORB1592789-100,BYT-ORB1592789-25,BYT-ORB1592789-50
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, ICC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human STIP1
Conjugation: Unconjugated
Alternative Names: MCM1, SRF_HUMAN, SRF, SRF_MOUSE,
STIP1 Recombinant Rabbit Monoclonal Antibody
Clonality: Recombinant
Concentration: 1mg/ml
Clone Designation: [B5C7]
Molecular Weight: 63 kDa
UniProt: P11831
Buffer: PBS, Glycerol, rAlbumin.
Form: Liquid
Target: STIP1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100
Cell line: HeLa, Fixation: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:500, Primary Ab incubation condition: 4C, overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1592789.
Cell line: HeLa, Fixation: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary Ab Dilution: 1:100, Secondary Ab: Goat Anti-Rabbit IgG, Unlabelled control: The cell without incubation, with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line).
Cell line: Neuro-2a, Fixation: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:500, Primary Ab incubation condition: 4C, overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1592789.
Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Rat Testis tissue lysates, Lane 4: Human A549 cell lysates, Lane 5: Human A431 cell lysates, Lane 6: Human 293T cell lysates, Lane 7: Human HepG2 cell lysates, Lane 8: Human Jurkat cell lysates, Primary: Anti-STIP1 (orb1592789) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63 kDa, Observed band size: 63 kDa.
Tissue: Human testis, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1592789.
Tissue: Rat testis, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1592789.