Anti-IFI16 antibody (orb1672345) was raised against a peptide corresponding to 16 amino acids near the carboxyl terminus of human IFI16. The immunogen is located within the last 50 amino acids of IFI16.
IFI16 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
IFI16
WB Validation in Human and Rat Cell Lines. Loading: 15 µg of lysate per lane. Antibodies: IFI16 orb1672345, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: IFI16 orb1672345, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Testis. Loading: 15 µg of lysate per lane. Antibodies: IFI16 orb1672345, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of IFI16 in Human Molt4 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 cells labeling IFI16 with orb1672345 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry Validation of IFI16 in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-IFI16 antibody (orb1672345) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of IFI16 in Mouse Liver. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-IFI16 antibody (orb1672345) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of IFI16 in Rat Testis. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-IFI16 antibody (orb1672345) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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