Western blot, 0.25-0.5 µg/ml, Human, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human Immunoprecipitation, 0.5-2 µg/ml, Human Flow Cytometry (Fixed), 1-3 µg/1x10 6 cells, Human EL
Flow Cytometry analysis of THP-1 cells using anti-Thrombomodulin/THBD antibody. Overlay histogram showing THP-1 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Thrombomodulin/THBD Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody and anti-Tubulin Alpha antibody. Thrombomodulin/THBD was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-Thrombomodulin/THBD Antibody and mouse anti-Tubulin Alpha antibody overnight at 4C. Cy3 Conjugated Goat Anti-Rabbit IgG and DyLight488 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody. Thrombomodulin/THBD was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Thrombomodulin/THBD Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody. Thrombomodulin/THBD was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Thrombomodulin/THBD Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Immunoprecipitating Thrombomodulin/THBD in A431 whole cell lysate. Western blot analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody. Lane 1: A431 whole cell lysates (30 ug) Lane 2: Rabbit control IgG instead of anti-Thrombomodulin/THBD antibody in A431 whole cell lysate. Lane 3: anti-Thrombomodulin/THBD antibody (2 µg) + A431 whole cell lysate (500 µg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Thrombomodulin/THBD antigen affinity purified polyclonal antibody at a dilution of 0.5 µg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for Thrombomodulin/THBD at approximately 100 kDa. The expected band size for Thrombomodulin/THBD is at 60 kDa.
Western blot analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane
IHC analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody. Thrombomod
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