0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form:
Liquid
Target:
CTH
Application Dilute:
WB=1:500-2000, Flow-Cyt=2ug/Test
Western blot analysis of Esophagus Mouse Lysate using CTH antibody.
Western blot analysis of Liver Mouse Lysate using CTH antibody.
Blank control: U937. Primary Antibody (green line): Rabbit Anti-CTH antibody (orb1688), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Sample: Esophagus (Mouse) Lysate at 40 ug, Primary: Anti-CTH (orb1688) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45kD, Observed band size: 45 kD.
Sample: K562 Cell (Human) Lysate at 40 ug, Primary: Anti-CTH (orb1688) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45kD, Observed band size: 45 kD.
Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-CTH (orb1688) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45kD, Observed band size: 48 kD.
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