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Rabbit KLF4 Recombinant Monoclonal Antibody, Clone: [BLR250L], Unconjugated

Rabbit KLF4 Recombinant Monoclonal Antibody, Clone: [BLR250L], Unconjugated

Catalog Number: BYT-ORB1710839

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Article Name:	Rabbit KLF4 Recombinant Monoclonal Antibody, Clone: [BLR250L], Unconjugated
Biozol Catalog Number:	BYT-ORB1710839
Supplier Catalog Number:	orb1710839
Alternative Catalog Number:	BYT-ORB1710839-10,BYT-ORB1710839-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	ICC, IHC, IP, WB
Species Reactivity:	Human, Mouse
Immunogen:	Between 75 and 125
Conjugation:	Unconjugated
Alternative Names:	endothelial Kruppel-like zinc finger protein, epithelial zinc finger protein EZF, EZF, GKLF, gut Kruppel-like factor, gut-enriched krueppel-like factor, Krueppel-like factor 4, Kruppel-like factor 4 (gut)

KLF4 Antibody

Clonality:	Recombinant
Concentration:	1000 µg/ml
Clone Designation:	[BLR250L]
NCBI:	004226
UniProt:	O43474
Buffer:	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Form:	Liquid
Target:	KLF4

Application Dilute:	WB: 1:1,000, IP: 6 µl/1 mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Application Notes:	Application Notes: Format: Whole IgG

	Detection of human KLF4 by immunocytochemistry. Sample: FFPE section of HCT 116 cells. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of mouse KLF4 by immunocytochemistry. Sample: FFPE section of CT26 cells. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of mouse KLF4 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human KLF4 by immunohistochemistry. Sample: FFPE section of human gastric carcinoma. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human KLF4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839) used for IP at 6 µl/mg lysate.
	Detection of human KLF4 by western blot. Samples: Whole cell lysate (25 µg) from DLD-1, PC-3, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
	Detection of mouse KLF4 by western blot. Samples: Whole cell lysate (25 µg) from CH27, CT26, C2C12, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

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