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Rabbit KLF5 Recombinant Monoclonal Antibody, Clone: [BLR243L], Unconjugated

Rabbit KLF5 Recombinant Monoclonal Antibody, Clone: [BLR243L], Unconjugated

Catalog Number: BYT-ORB1733253

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Article Name:	Rabbit KLF5 Recombinant Monoclonal Antibody, Clone: [BLR243L], Unconjugated
Biozol Catalog Number:	BYT-ORB1733253
Supplier Catalog Number:	orb1733253
Alternative Catalog Number:	BYT-ORB1733253-10,BYT-ORB1733253-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	FC, ICC, IHC, IP, WB
Species Reactivity:	Human, Mouse
Immunogen:	Between 325 and 375
Conjugation:	Unconjugated
Alternative Names:	basic transcription element binding protein 2, Basic transcription element-binding protein 2, BTEB2, BTE-binding protein 2, CKLF, colon krueppel-like factor, colon kruppel-like factor, epididymis secretory sperm binding protein, GC box binding protein 2, GC-box-binding protein 2, IKLF, intestinal Kruppel-like factor, intestinal-enriched krueppel-like factor, intestinal-enriched kruppel-like factor, Klf5C isoform, Krueppel-like factor 5, Kruppel-like factor 5 (intestinal), transcription factor BTEB2

KLF5 Antibody

Clonality:	Recombinant
Concentration:	1000 µg/ml
Clone Designation:	[BLR243L]
NCBI:	001721
UniProt:	Q13887
Buffer:	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Form:	Liquid
Target:	KLF5

Application Dilute:	Application : Flow-Cyt , ApplicationDilutionRange : Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.1 µl per 1 x 10 6 cells. , Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is r
Application Notes:	Application Notes: Format: Whole IgG

	Detection of mouse KLF5 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
	Detection of human KLF5 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
	Detection of human KLF5 by immunocytochemistry. Sample: FFPE section of HT-29 cells. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of mouse KLF5 by immunocytochemistry. Sample: FFPE section of CT26 cells. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human KLF5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of mouse KLF5 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human KLF5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from KM12 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253) used for IP at 6 µl/mg lysate.
	Detection of mouse KLF5 by western blot. Samples: Whole cell lysate (25 µg) from CT26, WEHI-231, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
	Detection of human KLF5 by western blot. Samples: Whole cell lysate (25 µg) from HeLa, SW620, MCF-7, and KM12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733253) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

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