solution in water, Color: colorless to slightly yellow, pH: 7.5 0.5
Formula:
C12H20N5O15P3
Application Notes:
Application Notes: Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription (up to 1400 bp tested). 3-Azide-Labeling of T7 promotor-containing oligonucleotides. The resulting azide-functionalized RNA can subsequently be processed via Cu(I)-free (azide-DBCO) or Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice: to introduce a Biotin group for subsequent purification tasks (via DBCO-functionalized Biotin or Alkynes of Biotin, respectively), to introduce fluorescent group for subsequent microscopic imaging (DBCO-functionalized fluorescent dyes or Alkynes of fluorescent dyes, respectively), to crosslink the RNA to azide-or alkyne functionalized biomolecules e.g.proteins. Spectroscopic Propertie: lambdamax 260 nm, epsilon 8.8 L mmol-1 cm-1 (Tris-HCl, pH 7.5)
Structural formula of 5-Azido-C3-UTP
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