Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with pH lowe
Target:
LGR5
Elisa plates were pre-coated with Flag Tag LGR5-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with LGR5-Nanodisc is 5.199 ng/ml.
Human LGR5-Nanodisc, Flag Tag on SDS-PAGE
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