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Rabbit EGFR Recombinant Monoclonal Antibody, Clone: [BLR252L], Unconjugated

Rabbit EGFR Recombinant Monoclonal Antibody, Clone: [BLR252L], Unconjugated

Catalog Number: BYT-ORB1784586

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Article Name:	Rabbit EGFR Recombinant Monoclonal Antibody, Clone: [BLR252L], Unconjugated
Biozol Catalog Number:	BYT-ORB1784586
Supplier Catalog Number:	orb1784586
Alternative Catalog Number:	BYT-ORB1784586-10,BYT-ORB1784586-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	ICC, IHC, IP, WB
Species Reactivity:	Human, Mouse
Immunogen:	Between 1160 and C-terminus
Conjugation:	Unconjugated
Alternative Names:	avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, epidermal growth factor receptor, epidermal growth factor receptor tyrosine kinase domain, ERBB, ERBB1, erb-b2 receptor tyrosine kinase 1, HER1, mENA, NISBD2, PIG61, proto-oncogene c-ErbB-1, receptor tyrosine-protein kinase erbB-1

Rabbit EGFR Recombinant Monoclonal Antibody

Clonality:	Recombinant
Concentration:	1000 µg/ml
Clone Designation:	[BLR252L]
NCBI:	958440
UniProt:	P00533
Buffer:	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Form:	Liquid
Target:	EGFR

Application Dilute:	Application : Flow-Cyt , ApplicationDilutionRange : Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 µl per 1 x 10 6 cells. , Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is
Application Notes:	Application Notes: Format: Whole IgG

	Detection of mouse EGFR (shaded) in mIMCD-3 cells by flow cytometry. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
	Detection of human EGFR (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
	Detection of human EGFR by immunocytochemistry. Sample: FFPE section of A-431 cells. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human EGFR by immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human EGFR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP, 10% of IP loaded) from OVCAR-8 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586) used for IP at 6 µl per reaction.
	Detection of human EGFR by western blot. Samples: Whole cell lysate (10 µg) from GaMG, HeLa, OVCAR-8, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
	Detection of mouse EGFR by western blot. Samples: Whole cell lysate (10 µg) from AML12, NIH 3T3, and mIMCD-3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

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