Anti-IL-31Ra Reference Antibody is expressed from CHO. The heavy chain type is huIgG2, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
Anti-IL-31Ra Reference Antibody (nemolizumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Anti-IL-31Ra Reference Antibody (nemolizumab)is more than 100% ,determined by SEC-HPLC.
Immobilized human IL31R His at 2 µg/mL can bind Anti-IL-31Ra Reference Antibody (nemolizumab),EC50=0.0153µg/mL.
Human IL31R HEK293 cells were stained with Anti-IL-31Ra Reference Antibody (nemolizumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC181=0.307 µg/mL.
Anti-IL-31Ra Reference Antibody (nemolizumab) P-STAT3 Assay was evaluated using Du145. The IC50 was approximately 0.1068 ug/ml.
Anti-IL-31Ra Reference Antibody (nemolizumab) Activation inhibition was evaluated using Hacat. The max induction fold was approximately 1.86.
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