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Rabbit Met Recombinant Monoclonal Antibody, Clone: [BLR283L], Unconjugated

Rabbit Met Recombinant Monoclonal Antibody, Clone: [BLR283L], Unconjugated

Catalog Number: BYT-ORB1806454

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Article Name:	Rabbit Met Recombinant Monoclonal Antibody, Clone: [BLR283L], Unconjugated
Biozol Catalog Number:	BYT-ORB1806454
Supplier Catalog Number:	orb1806454
Alternative Catalog Number:	BYT-ORB1806454-10,BYT-ORB1806454-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	FC, ICC, IHC, IP, WB
Species Reactivity:	Human
Immunogen:	Between 1340 and C-terminus
Conjugation:	Unconjugated
Alternative Names:	AUTS9,c-Met,DFNB97,hepatocyte growth factor receptor,HGF receptor,HGF/SF receptor,HGFR,proto-oncogene c-Met,RCCP2,scatter factor receptor,SF receptor,tyrosine-protein kinase Met

Rabbit Met Recombinant Monoclonal Antibody

Clonality:	Recombinant
Concentration:	1000 µg/ml
Clone Designation:	[BLR283L]
NCBI:	000236
UniProt:	P08581
Buffer:	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Form:	Liquid
Target:	Met

Application Dilute:	Application : Flow-Cyt , ApplicationDilutionRange : Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 µl per 1 x 10 6 cells. , Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is r
Application Notes:	Application Notes: Format: Whole IgG

	Detection of human MET (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
	Detection of human MET by immunocytochemistry. Sample: FFPE section of GaMG cells. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human MET by immunocytochemistry. Sample: FFPE section of NCI-H226 cells. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human MET by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human MET by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human MET by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP, 10% of IP loaded) from GaMG cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MET recombinant monoclonal antibody (orb1806454) used for IP at 6 µl/mg lysate.
	Detection of human MET by western blot. Samples: Whole cell lysate (10 µg) from GaMG, T-47D, HT-29, OVCAR-8, and 786-O cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

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