NGFR/p75NTR Recombinant Rabbit Monoclonal Antibody, Clone: [5D4], Unconjugated
Catalog Number:
BYT-ORB1816952
- Images (5)
| Article Name: | NGFR/p75NTR Recombinant Rabbit Monoclonal Antibody, Clone: [5D4], Unconjugated |
| Biozol Catalog Number: | BYT-ORB1816952 |
| Supplier Catalog Number: | orb1816952 |
| Alternative Catalog Number: | BYT-ORB1816952-100,BYT-ORB1816952-25,BYT-ORB1816952-50 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ICC, IF, IHC-Fr, IHC-P |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | A synthesized peptide derived from human p75 NTR (350-400aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR, LNGFR, p75, p75NGFR, RNNGFRR, TNR16_HUMAN, NGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor (NGF receptor), Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR, p75 ICD, TNR16_MOUSE, TNR16_RAT, |
| NGFR/p75NTR Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [5D4] |
| Molecular Weight: | 44 kDa |
| UniProt: | P08138 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | NGFR |
| Application Dilute: | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500 |
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Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: SW480, 2: PC-12, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 45 kDa, Observed MW: 70 kDa. |
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Cell line: PC-12, Fixation: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary Ab incubation condition: 4C, overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1816952. |
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Paraformaldehyde-fixed, paraffin embedded (human esophagus), Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NGFR) Monoclonal Antibody, Unconjugated (orb1816952) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human spleen), Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NGFR) Monoclonal Antibody, Unconjugated (orb1816952) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NGFR) Monoclonal Antibody, Unconjugated (orb1816952) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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