The Donkey Anti-Rabbit IgG H&L, BF647 conjugated is a BF647 conjugated polyclonal antibody suitable for FC, ICC, IF. This product reacts with Rabbit samples.
Clonality:
Polyclonal
Concentration:
2.0 mg/ml
Buffer:
10 mM TBS (pH=7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% glycerol.
Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (orb500829) at 1:200 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb1817041) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (orb500829) at 1:200 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb1817041) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (orb500829) at 1:200 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb1817041) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (orb500829) at 1:200 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb1817041) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (orb500829) at 1:200 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb1817041) for 90 minutes, and DAPI for nuclei staining.
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