Erk1/2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1822436
Article Name: Erk1/2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1822436
Supplier Catalog Number: orb1822436
Alternative Catalog Number: BYT-ORB1822436-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, ICC, IF, IHC, WB
Species Reactivity: Bovine, Drosophila, Frog, Gallus, Human, Mouse, Rat, Sheep
Immunogen: A 35 residue synthetic peptide, corresponding to Rat Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
Conjugation: Unconjugated
Alternative Names: ERK1, ERK2, ERT1, ERT2, MAP kinase 1, MAP kinase 2, MAPK1, MAPK2, MAPK3, p38, p40, p41, p41mapk, p42 MAPK, p44 ERK1, p44 MAPK, PRKM1, PRKM2, PRKM3
Rabbit Anti-Rat Erk1/2 Polyclonal
Clonality: Polyclonal
Concentration: 1 mg/ml
Molecular Weight: 42kDa (ERK2).
NCBI: 059043
UniProt: P21708
Buffer: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
Target: Erk1/2
Application Dilute: WB (1:1000), IHC (1:100), ICC/IF (1:100), FCM (1:100), optimal dilutions for assays should be determined by the user.
Application Notes: Application Notes: A 1:1000 dilution was sufficient for detection of ERK1/2 in 20 µg of HeLa cell lysate by ECL immunoblot analysis
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Erk1/2 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.
Western blot analysis of Human Cell line lysates showing detection of ERK1 protein using Rabbit Anti-ERK1 Polyclonal Antibody. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody. Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.
Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative Solution. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Erk1/2 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ERK1 Polyclonal Antibody. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cytoplasm. Nucleus.
510,00 €
100 ul
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